Properties | ||
DNA Molecular Weight | Calculate the molecular weight of DNA; single-stranded sequences are counted with a 5'-P. | |
Reverse Complement | Convert a DNA sequence into its reverse, complement, or reverse-complement. | |
Alignments | ||
Pairwise DNA Alignment | Align two DNA sequences and color the resulting global alignment. | |
ORFs, Codons & Translation | ||
Translation Map | Generate a textual representation of DNA translation in varied reading frames. | |
Translate | Translate one or more provided DNA sequence in the desired reading frames. | |
Back Translate | Back Translate generates a DNA sequence representing the most likely non-degenerate coding sequence from a protein sequence and draw the corresponding translation map. | |
Codon Usage | Calculate the number and frequency of each codon. | |
ORF Finder | Search for open reading frames (ORFs) in the provided DNA sequences. | |
Transseq | Translate DNA in varied formats (GCG, FASTA, EMBL, GenBank, PIR, NBRF or Phylip). | |
Translate DNA | Online tool with varied graphical output options. | |
Restriction Enzymes | ||
Restriction Map | Create a sequence map showing the positions of restriction endonuclease cut sites. | |
Restriction Summary | Summary of number and positions of commonly used restriction endonuclease cut sites. | |
Restriction Digest | Determine the DNA sequences resulting from a restriction digest. | |
PCR and Primers | ||
Primer Map | Create a sequence map showing annealing positions of PCR primers, protein translation, and common restriction sites. | |
PCR Products | Generate the PCR product from a DNA sequence and 2 primers. | |
Oligonucleotide Analysis | Determine standard primer properties, including melting temperature, percent GC content, and PCR suitability. | |
Sequence Statistics, Patterns & Motifs | ||
DNA Pattern Search | Locate DNA patterns and motifs along the two strands of a DNA sequence. | |
CpG Islands | Detect potential CpG island regions using a 200 bp window according to the method of Gardiner-Garden and Frommer (1987). | |
DNA Stats | DNA Stats analyzes occurrences and frequency of each nucleotide and dinulceotides in the sequence. | |
Random Sequences | ||
Random DNA | Generates a random DNA sequence. | |
Random Coding DNA | Generates a random coding DNA sequence. | |
Shuffle DNA | Randomly shuffles a DNA sequence. | |
Seqmake | Online tool to generate random sequences. | |
Format Conversion | ||
Genbank to FASTA | Convert a Genbank-formatted DNA sequence in FASTA format. | |
EMBL to FASTA | Convert an EMBL-formatted DNA sequence in FASTA format. | |
Genbank Feature Extractor | Extract sequence features from a Genbank-formatted DNA sequence. | |
EMBL Feature Extractor | Extract sequence features from an EMBL-formatted DNA sequence. | |
Filter DNA | Removes non-DNA characters from text including text marks, numbers, and spaces. | |
Format DNA | Format DNA sequence output with selected line length, segments, and base numbering. | |
Combine FASTA | Assemble multiple FATA sequences in a single sequence. |
Properties | ||
Protein Molecular Weight | Convert a Genbank-formatted DNA sequence in FASTA format. | |
Protein Isoelectric Point | Calculate the theoretical isoelectric point of a protein sequence. | |
Protein GRAVY | Calculate the GRAVY (grand average of hydropathy) value for a protein sequence. | |
Alignments | ||
Pairwise Protein Alignment | Align two protein sequences and color the resulting global alignment. | |
Protein Statistics, Patterns & Motifs | ||
Protein Pattern Search | Locate patterns and motifs along the polypeptide sequence of a protein. | |
Protein Stats | Calculate the number of occurrences of each residue in a polypeptide sequence. | |
Random Sequences | ||
Random Protein | Generate random protein sequences. | |
Shuffle Protein | Shuffle protein sequences. | |
RandSeq | Online tool to generate a random protein sequence. | |
Format Conversion | ||
GenBank Trans Extractor | Extract each of the protein translations described in a Genbank file. | |
EMBL Trans Extractor | Extract each of the protein translations described in an EMBL file. | |
Three to One | Convert three letter translations to single letter translations. | |
One to Three | Convert single letter translations to three letter translations. | |
Filter Protein | Removes non-protein characters from sequence text including text marks, numbers, and spaces. | |
Format Protein | Format protein sequence with specific group size, line length and adds numbering. |